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Updated: Jul 18, 2026
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Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
Published on: May 16, 2025
Methodological improvements on extraction of nuclear proteins and its preliminary analysis during the maize (Zea mays
B S Ferreira1, A T Branco, M A de Oliveira
1Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro / Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil. ferreirabs@uenf
Protein and Peptide Letters
|December 16, 2006
Abstract:
A procedure to obtain endosperm protein extracts was standardized. After confirming the enrichment with nuclear proteins by immunodetection, the protein profiles of extracts from different seed development stages were compared by SDS-PAGE that showed the existence of several differentially expressed proteins.