Improved endocervical sampling and HPV viral load detection by Cervex-Brush Combi

Area of Science:

• Gynecologic Cytopathology
• Molecular Diagnostics
• Cervical Cancer Screening

Background:

• Liquid-based cytology (LBC) is increasingly used for cervical screening.
• The Cervex-Brush is a common device for sample collection in SurePath LBC.
• The Rovers Cervex-Brush Combi is a novel device designed to improve endocervical cell sampling.

Purpose of the Study:

• To compare the Cervex-Brush Combi with the Cervex-Brush for cell collection.
• To evaluate HPV typing/quantification and disease detection using SurePath LBC.
• To assess the efficacy of the Cervex-Brush Combi in improving sample adequacy.

Main Methods:

• 200 SurePath LBC samples were collected using either the Cervex-Brush or Cervex-Brush Combi.
• Slides were screened using guided screening with slide wizards.
• HPV viral load was determined using TaqMan-based real-time quantitative PCR.

Main Results:

• No significant difference in squamous cell collection between brush types.
• Cervex-Brush Combi yielded a 2-3 fold increase in endocervical cells (P < 0.00001).
• Increased detection of abnormal smears and higher HPV viral loads observed with Cervex-Brush Combi.

Conclusions:

• Cervex-Brush Combi enhances endocervical cell collection compared to Cervex-Brush.
• This improved sampling leads to higher detection rates of oncogenic HPV and abnormal cervical lesions.
• The Cervex-Brush Combi is a valuable tool for improving cervical cancer screening efficacy.