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Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Analysis of Pneumocystis carinii gene function by complementation in yeast mutants

Philippe M Hauser1, Libera Lo Presti, Moira Cockell

  • 1Microbiology Institute, University Hospital, 1011 Lausanne, Switzerland. Philippe.Hauser@chuv.ch

The Journal of Eukaryotic Microbiology
|December 16, 2006
PubMed
Abstract

No abstract available in PubMed .

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Complementation Tests00:49

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...