Time course of receptor-channel coupling in frog sympathetic neurons

The M-type potassium current and the N-type calcium current are inhibited by several different neurotransmitters in frog sympathetic neurons. These effects seem to be mediated via G proteins, but it is not clear whether diffusible second messengers are involved. Using a rapid (approximately 100 ms) flow tube perfusion system to apply agonists, the inhibition of calcium current develops and recovers rapidly but not instantaneously (t1/2 = 1-2 s). M-current inhibition is considerably slower, with t1/2 approximately 30 s for recovery from inhibition. At least for M-current inhibition, there appears to be sufficient time for involvement of an enzymatic cascade in receptor-channel coupling.