Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development-specific genes and their expression at the RNA level.

Z H Jin1, Z R Sung

  • 1Institute of Developmental Biology, Academic Sinica, Beijing.

Chinese Journal of Biotechnology
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strengthening Ni alloys with nanoscale interfaces of negative excess energy.

Science (New York, N.Y.)·2025
Same author

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste.

Letters in applied microbiology·2021
Same author

Constrained minimal-interface structures in polycrystalline copper with extremely fine grains.

Science (New York, N.Y.)·2020
Same author

[Effects of N-(4-hydroxyphenyl) retinamide lipid microbubble combined with ultrasound on human keloid fibroblasts].

Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns·2018
Same author

Paracrine regulation of melanogenesis.

The British journal of dermatology·2017
Same author

Disaster nursing experiences of Chinese nurses responding to the Sichuan Ya'an earthquake.

International nursing review·2016

Researchers identified new carrot cDNA genes crucial for somatic embryogenesis. Clone 22 shows specific developmental regulation, closely linked to carrot somatic embryo development.

Area of Science:

  • Molecular Biology
  • Plant Science
  • Biotechnology

Background:

  • Somatic embryogenesis is a key process in plant biotechnology for asexual propagation.
  • Understanding the genetic regulation of somatic embryogenesis is crucial for improving crop development.

Purpose of the Study:

  • To identify and characterize novel genes involved in carrot (Daucus carota) somatic embryogenesis.
  • To investigate the tissue-specific and temporal expression patterns of these candidate genes.

Main Methods:

  • Construction and screening of a cDNA expression library using phage lambda gt11.
  • DNA isolation, restriction enzyme digestion (EcoRI), gel electrophoresis, and subcloning into plasmid vectors (pIBI, pUC18).
  • Southern blot analysis for cross-hybridization and Northern blot analysis for gene expression profiling.

Related Experiment Videos

Main Results:

  • Seven specific clones for carrot somatic embryo development were isolated.
  • Four clones (16, 22, 50, and 60) were identified as novel cDNA genes through Southern blot analysis.
  • Northern blot analysis revealed tissue-specific expression in various carrot organs and time-course expression in callus and embryo cells, with clone 22 exhibiting developmental regulation linked to embryogenesis.

Conclusions:

  • Novel cDNA genes associated with carrot somatic embryogenesis have been identified.
  • Clone 22 represents a specific gene regulated by development and closely involved in carrot somatic embryogenesis.