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Updated: Aug 18, 2026
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Published on: December 1, 2015
Immunohistochemical evaluation of reverse transcriptase in breast carcinoma with polyclonal antibodies raised in
C F Moyret1, D J Bernard, J C Maurizis
1Laboratoire d'Immunologie et de Cancérologie, Centre Jean Perrin, Clermont-Ferrand, France.
Abstract:
In this study, the presence of reverse transcriptase in breast tumours was examined with immunoperoxidase staining using antibodies raised in rabbit against reverse transcriptase of Moloney murine leukemia virus and against reverse transcriptase of avian myeloblastosis virus. The specificity of such antibodies was investigated with ELISA and Western blotting techniques. Five cases of infiltrating ductal carcinomas were found positive with the immune serum anti-reverse transcriptase of Moloney murine leukemia virus on 28 studied infiltrating ductal carcinomas, 2 infiltrating lobular carcinomas and 2 fibroadenomas.
