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Updated: Jul 18, 2026

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
08:43

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Published on: February 14, 2017

Complement and leukocytes during cardiopulmonary bypass: effects on plasma C3d and C5a, leukocyte count, release of

F Knudsen1, J O Pedersen, O Juhl

  • 1Department of Anesthesia and Intensive Care, Aalborg Hospital, Denmark.

Journal of Cardiothoracic Anesthesia
|April 1, 1988
PubMed

Insights

Cardiopulmonary bypass (CPB) triggers an acute inflammatory response, altering complement-leukocyte systems. This study observed changes in complement factors and impaired granulocyte migration during CPB.

Area of Science:

  • Immunology
  • Cardiovascular Surgery
  • Hematology

Background:

  • Cardiopulmonary bypass (CPB) is associated with complex inflammatory responses.
  • Understanding the complement-leukocyte system's role during CPB is crucial for patient outcomes.

Purpose of the Study:

  • To investigate changes in complement activation and leukocyte function during CPB.
  • To elucidate the impact of CPB on the complement-leukocyte system and granulocyte migration.

Main Methods:

  • Plasma levels of C3d, C5a, and granulocyte elastase bound to alpha1-proteinase inhibitor (E-alpha1 PI) were measured.
  • Leukocyte counts, differential cell counts, and granulocyte migration were assessed before, during, and after CPB.
  • Hemodilution was corrected for in plasma C3d measurements.

Main Results:

  • Complement activation was confirmed by increased plasma C3d levels during CPB.
  • Significant C5a levels were not detected.
  • Plasma E-alpha1 PI increased, indicating granulocyte lysosomal enzyme release.
  • Granulocyte migration was transiently impaired due to an intrinsic cellular defect.
  • Leukocyte counts decreased during CPB but remained unchanged after correcting for hemodilution, except for a slight lymphocyte decrease.
  • Post-CPB, neutrocytosis and lymphopenia were observed.

Conclusions:

  • CPB induces acute changes in plasma and cells, mimicking a systemic inflammatory response.
  • Transient impairment of granulocyte migration occurs during CPB.
  • The clinical significance of these CPB-induced inflammatory changes requires further investigation.

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