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Updated: Jul 18, 2026

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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in
Régis Hallez1, Jean-Jacques Letesson, Jean Vandenhaute
1Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium.
Applied and Environmental Microbiology
|December 19, 2006
Abstract:
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

