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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
Published on: October 17, 2017
Proteomic analysis of foam cells.
Peng-Yuan Yang1, Yao-Cheng Rui, Peng-Yuan Yang
1Department of Pharmacology, Secondary Military Medical University, Shanghai, China.
Methods in Molecular Biology (Clifton, N.J.)
|December 19, 2006
Summary
Researchers studied macrophage-derived foam cells, key in atherosclerosis, by analyzing protein expression changes. This research identifies potential protein markers for inflammatory reactions in atherosclerotic studies.
Area of Science:
- Biochemistry
- Cell Biology
- Proteomics
Background:
- Foam cells are characteristic pathological cells in atherosclerotic lesions.
- Macrophage-derived foam cell models are crucial for studying atherosclerosis.
- Previous studies have analyzed protein expression profiles in foam cells.
Purpose of the Study:
- To establish and analyze an in vitro macrophage-derived foam cell model.
- To investigate global protein expression changes in U937 foam cells compared to normal U937 cells.
- To identify potential protein markers for inflammatory reactions in atherosclerotic studies.
Main Methods:
- Established an in vitro foam cell model by incubating human U937 cells with oxidized low-density lipoprotein.
- Measured global protein expression changes using two-dimensional gel electrophoresis.
- Identified proteins via mass spectrometry after capillary liquid chromatography separation and tryptic digestion.
- Validated identified proteins using the U937 proteomic map from the Expasy Proteomics server.
Main Results:
- Global protein expression profiles were compared between U937 foam cells and normal U937 cells.
- Several proteins of interest were identified through mass spectrometry.
- The experimental data provides a basis for identifying potential inflammatory markers.
Conclusions:
- The study successfully established and characterized an in vitro foam cell model.
- The proteomic analysis revealed significant protein expression changes.
- The findings offer potential protein markers for understanding inflammatory processes in atherosclerosis.
