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Updated: Jul 18, 2026
MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
Guidelines for the selection of effective short-interfering RNA sequences for functional genomics
Kumiko Ui-Tei1, Yuki Naito, Kaoru Saigo
1Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan.
Abstract:
To avoid long-double-stranded-RNA-dependent interferon response, short-interfering RNAs (siRNAs) are widely used for RNA interference (RNAi) in mammalian cells. siRNA-based RNAi, however, may not be readily available for the large-scale gene silencing essential for systematic functional genomics, because only a limited fraction of siRNAs is capable of inducing effective mammalian RNAi. siRNAs correctly designed for the knockdown of a particular gene may also destroy the functions of unrelated genes. Here, we describe algorithms by which these serious setbacks can be eliminated in mammalian functional genomics using RNAi and a Web-based online software system for computing highly functional siRNA sequences with maximal target-specificity in mammalian RNAi.
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