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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: Jul 18, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

Target validation using RNA interference in solid tumors.

Seyedhossein Aharinejad¹, Mouldy Sioud, Trevor Lucas

¹Laboratory for Cardiovascular Research, Center for Anatomy and Cell Biology, Vienna Medical University, Vienna, Austria.

Methods in Molecular Biology (Clifton, N.J.)

|December 19, 2006

Summary

Suppressing colony-stimulating factor-1 (CSF-1) using RNA interference effectively inhibits tumor growth and metastasis. This approach impacts tumor-stroma interactions, reducing vascularity and macrophage recruitment in solid tumors.

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Area of Science:

Oncology
Molecular Biology
Immunology

Background:

Reverse genetics links gene function to disease phenotypes by inhibiting gene expression.
Tumor growth and metastasis involve complex interactions between cancer cells, stroma, and extracellular matrix (ECM).
Colony-stimulating factor-1 (CSF-1) regulates macrophage production and is often overexpressed in tumors.

Purpose of the Study:

To investigate the efficacy of inhibiting colony-stimulating factor-1 (CSF-1) using RNA interference (RNAi) in blocking tumor growth.
To explore the impact of CSF-1 suppression on tumor-stroma interactions, angiogenesis, and macrophage recruitment.

Main Methods:

Utilized short-interfering RNAs (siRNAs) to target CSF-1 or its receptors in colon and breast cancer xenografts in mice.
Monitored tumor growth, vascularity, angiogenic factors, matrix metalloproteases (MMPs), and macrophage infiltration.

Main Results:

Inhibition of CSF-1 significantly suppressed tumor growth and metastasis in mouse models.
Observed decreased tumor vascularity, reduced expression of angiogenic factors and MMPs.
Demonstrated reduced macrophage recruitment to the tumor site following CSF-1 suppression.

Conclusions:

RNA interference targeting CSF-1 is a potent strategy for inhibiting gene function in cancer.
Suppression of CSF-1 effectively disrupts tumor-stroma interactions, impacting angiogenesis and tumor development.
This approach holds promise for novel therapeutic strategies against solid tumors.