Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tagging and detection strategies for activity-based proteomics.

Amir M Sadaghiani1, Steven Hl Verhelst, Matthew Bogyo

  • 1Department of Pathology, Stanford University, Stanford, CA 94305-5324, USA.

Current Opinion in Chemical Biology
|December 19, 2006
PubMed
Summary

Activity-based probes (ABPs) enable monitoring of enzyme activity, not just protein levels. Recent advances in probe diversity and detection methods, including fluorophores and bioorthogonal chemistry, expand their applications in proteomics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging of <i>Staphylococcus aureus</i> infections and biofilms using a selective covalent probe for the unique serine hydrolase FphE.

bioRxiv : the preprint server for biology·2026
Same author

Imaging of Staphylococcus aureus Infections and Biofilms Using a Selective Covalent Probe for the Unique Serine Hydrolase FphE.

Angewandte Chemie (International ed. in English)·2026
Same author

A broad cathepsin inhibitor blocks crystal-stimulated inflammasome-dependent and -independent inflammation and gout arthritis.

Communications medicine·2026
Same author

An Optimized Route to the Syringolin Natural Products Enables Combinatorial Synthesis of Selective, Bioactive Inhibitors of the <i><i>Plasmodium falciparum</i></i> 20S Proteasome.

Journal of medicinal chemistry·2026
Same author

Unique structural and ligand-binding properties of the <i>Staphylococcus aureus</i> serine hydrolase FphE.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Mycobacteriophage Functionalized Magnetic Nanocrystal Clusters for Highly Sensitive and Rapid Detection of <i>Mycobacterium tuberculosis</i>.

JACS Au·2025

Area of Science:

  • Biochemistry
  • Proteomics
  • Chemical Biology

Background:

  • Activity-based proteomics utilizes small molecules called activity-based probes (ABPs) to tag and monitor specific proteins.
  • ABPs enable the analysis of systems-wide changes at the enzyme activity level, complementing traditional protein abundance studies.

Purpose of the Study:

  • To review the advancements in activity-based proteomics.
  • To highlight the expanding diversity of activity-based probes and their applications.

Main Methods:

  • Development of diverse activity-based probe families.
  • Utilization of small organic fluorophores for probe visualization.
  • Application of bioorthogonal coupling chemistries for post-labeling modifications.

Related Experiment Videos

Main Results:

  • Increased number and types of enzymes targetable by ABPs.
  • Enhanced methods for visualizing probes bound to target enzymes.
  • Expansion of ABP applications from biochemical profiling to in vivo imaging.

Conclusions:

  • Activity-based proteomics has rapidly evolved with diverse probe development.
  • Small organic fluorophores and bioorthogonal chemistry have significantly broadened ABP applications.
  • ABPs are powerful tools for studying enzyme activity in complex biological systems and in vivo.