Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

What can myosin VI do in cells?

H Lee Sweeney1, Anne Houdusse

  • 1Department of Physiology University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104-6085 USA. Lsweeney@mail.med.upenn.edu

Current Opinion in Cell Biology
|December 19, 2006
PubMed
Summary

The myosin VI motor

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combination S100A1 and ARC gene therapy as a treatment for DMD cardiomyopathy.

JCI insight·2026
Same author

Dynamics of the β-cardiac myosin auto-inhibited state explain cardiomyopathy pathogenesis.

Nature communications·2026
Same author

Direct Observation of Nanometer-Sized Steps of Single Myosin VI Molecules in Living Cells.

Nano letters·2026
Same author

Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophy.

Skeletal muscle·2026
Same author

Hypertrophic cardiomyopathy mutations Y115H and E497D disrupt the folded-back state of human β-cardiac myosin allosterically.

Nature communications·2025
Same author

Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophy.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Myosin VI is a unique motor protein with unconventional directionality.
  • Understanding its structure-function relationship is key to elucidating its cellular roles.

Purpose of the Study:

  • To investigate the structural basis for myosin VI's reversed directionality and unique kinetic properties.
  • To explore how myosin VI's structural features enable its dual roles as a transporter and anchoring protein.

Main Methods:

  • X-ray crystallography to determine the structure of the myosin VI motor.
  • Biochemical and biophysical assays to study kinetic properties and dimerization.
  • Cell biological studies to observe in vivo functions.

Main Results:

  • A unique insert in the myosin VI structure reverses its normal directionality.
  • A second insert near the nucleotide-binding pocket tunes its kinetics for dual functions.
  • Myosin VI forms dimers upon clustering and functions in both transport and anchoring.

Conclusions:

  • Myosin VI's unique structural elements dictate its unconventional function.
  • These adaptations allow myosin VI to fulfill specialized roles within cells.

Related Experiment Videos