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Related Experiment Videos

Hepatitis E virus: identification of type-common epitopes.

P O Yarbough1, A W Tam, K E Fry

  • 1Molecular Virology Department, Genelabs Incorporated, Redwood City, California 94063.

Journal of Virology
|November 1, 1991
PubMed
Summary

Researchers isolated two cDNA clones from the Mexico strain of hepatitis E virus (HEV). These clones encode epitopes crucial for diagnosing HEV infection and offer insights into HEV

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Area of Science:

  • Virology
  • Hepatology
  • Molecular Biology

Background:

  • Large outbreaks of enterically transmitted non-A, non-B viral hepatitis (ET-NANBH) are prevalent in developing nations.
  • The causative agent, hepatitis E virus (HEV), has a recently described molecular clone.

Purpose of the Study:

  • To isolate and characterize cDNA clones from the Mexico strain of HEV.
  • To investigate the diagnostic potential of these clones for HEV infection.

Main Methods:

  • Serologic screening of fecal samples from a 1986 ET-NANBH outbreak in Mexico.
  • Isolation and characterization of two HEV-derived cDNA clones.
  • Expression of recombinant fusion proteins and antibody reactivity testing.
  • Hybridization studies with HEV genomic RNA and comparison with the Burma strain.

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Main Results:

  • Two cDNA clones encoding HEV epitopes were successfully isolated from the Mexico strain.
  • These clones reacted specifically with sera from ET-NANBH patients and experimentally infected macaques.
  • HEV transcripts of approximately 2.0 and 3.7 kb were identified in infected liver tissue.
  • Amino acid homologies of 90.5% and 73.5% were found between Mexico and Burma HEV strains in ORF2 and ORF3, respectively.

Conclusions:

  • The isolated cDNA clones provide valuable tools for HEV diagnosis.
  • These findings offer insights into the expression strategy of the hepatitis E virus.
  • The study highlights significant genetic diversity between HEV strains.