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[Protective effect of recombinant beta-defensin-2 on acute lung injury induced by sepsis in rats]
Zhuo Shi1, Zheng-yan Zhao, Qiang Shu
1The Affiliated Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Objective:
To explore the protective effect of recombinant beta-defensin-2 (BD2) on acute lung injury induced by sepsis in rats.
Methods:
Recombinant adenovirus carrying an expression cassette of rat BD2 (rBD2) or control adenovirus carrying empty vector was administered intratracheally to Sprague-Dawley rats 48 h before acute lung injury, which was induced by cecal ligation and double puncture (CLP). Histological changes of the lungs and 7-day survival rate were determined.
Result:
The alveolar damage, interstitial edema, and infiltration of neutrophils were reduced and the survival rate was increased significantly in BD2 group than those in control group (P < 0.05). The CFU of abdominal bacteria was comparable to that in the control rats (P > 0.05).
Conclusion:
The administration of recombinant BD2 protects lung injury and improves the survival of rats with induced sepsis.