Related Experiment Video
Updated: Jul 15, 2026
07:22
Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
Published on: November 20, 2013
A renewable nanosensor based on a glass nanopipette.
Joe D Piper1, Richard W Clarke, Yuri E Korchev
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom.
Journal of the American Chemical Society
|December 21, 2006
Summary
A novel fluorescent nanosensor was developed for rapid, localized chemical measurements. This 100 nm device accurately maps pH and sodium ion concentrations with millisecond precision.
Area of Science:
- Nanotechnology
- Chemical Sensing
- Analytical Chemistry
Background:
- Accurate and rapid measurement of local chemical environments is crucial for understanding biological and chemical processes.
- Existing sensing technologies often lack the required spatial or temporal resolution for dynamic microscale analyses.
Purpose of the Study:
- To develop a novel fluorescent nanosensor for high-resolution chemical measurements.
- To demonstrate the nanosensor's capability for simultaneous pH and sodium ion concentration mapping.
Main Methods:
- Development of a 100 nm sized nanosensor utilizing reporter dye molecules encapsulated within a nanopipette tip.
- Integration of fluorescence detection for signal readout.
- Calibration and validation of the nanosensor for pH and sodium ion measurements.
Main Results:
- The developed nanosensor achieved a size of 100 nm.
- Successful measurement of local pH with high accuracy.
- Effective mapping of sodium ion concentration.
- Demonstrated temporal resolution in the millisecond range for both measurements.
Conclusions:
- The novel fluorescent nanosensor offers a powerful tool for microscale chemical analysis.
- The device provides unprecedented temporal resolution for dynamic sensing applications.
- This technology has potential applications in various fields requiring precise chemical monitoring.
