Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
¹H NMR Signal Multiplicity: Splitting Patterns01:13

¹H NMR Signal Multiplicity: Splitting Patterns

When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Novel Cloxyquin Analogs for K<sub>2P</sub>18.1 Channel Modulation.

Archiv der Pharmazie·2026
Same author

Noncanonical ion channel signaling in neurovascular barrier regulation and immune cell trafficking.

Current opinion in immunology·2026
Same author

Light-responsive aggregation of vesicles using host-guest interaction of β-cyclodextrin and diazocine.

Chemical communications (Cambridge, England)·2026
Same author

Photoisomerization and Electrochemical Switching of Arylazopyrazoles in Self-Assembled Monolayers.

ACS applied materials & interfaces·2026
Same author

Photochromic Cholesteric Liquid Crystals via Arylazopyrazole Functionalization of Hydroxypropyl Cellulose.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Self-Assembled Monolayers of Diazocine Photoswitches: Inverted Isomer Stability and Surface Wettability.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Jul 14, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Expression of a supramolecular complex at a multivalent interface.

Olga Crespo-Biel1, Choon Woo Lim, Bart Jan Ravoo

  • 1Laboratories for Supramolecular Chemistry & Technology and Molecular Nanofabrication, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

Journal of the American Chemical Society
|December 21, 2006
PubMed
Summary

Researchers explored multivalent binding using cyclodextrin (CD) host-guest and metal ion coordination. They achieved significant binding enhancement on surfaces, demonstrating a powerful supramolecular strategy for advanced materials.

More Related Videos

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
10:36

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Published on: June 15, 2021

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jul 14, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
10:36

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Published on: June 15, 2021

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

  • Supramolecular chemistry
  • Surface chemistry
  • Coordination chemistry

Background:

  • Multivalent interactions enhance binding affinity.
  • Orthogonal host-guest and coordination chemistry offer versatile molecular assembly tools.
  • Surface-based assays are crucial for studying binding phenomena at interfaces.

Purpose of the Study:

  • To describe the multivalent binding of supramolecular complexes to a multivalent host surface.
  • To combine orthogonal beta-cyclodextrin (CD) host-guest and metal ion-ethylenediamine coordination motifs.
  • To quantify binding enhancement at interfaces using a heterotropic multivalent binding model.

Main Methods:

  • Utilized adamantyl-functionalized ethylenediamine derivatives as divalent linkers.
  • Complexed linkers with Cu(II) or Ni(II) metal ions.
  • Studied binding to a CD self-assembled monolayer (SAM) using surface plasmon resonance (SPR) spectroscopy as a function of pH.

Main Results:

  • Quantified multivalent enhancement at the surface using a heterotropic binding model.
  • Observed a binding enhancement factor >100 for the Cu(II) complex on the CD surface compared to solution.
  • Confirmed divalent binding for both Cu(II) and Ni(II) systems on CD SAMs, despite potential for trivalent binding in the Ni(II) case.

Conclusions:

  • Demonstrated successful combination of CD host-guest and metal coordination for multivalent surface binding.
  • Achieved significant binding enhancement on surfaces through supramolecular complexation.
  • Validated the utility of SPR and a multivalent binding model for characterizing interfacial interactions.