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[Determination of tuberculosis antibodies using an immunoenzyme assay in lyophilized samples of capillary blood]
Laboratornoe Delo
|January 1, 1991
Abstract:
The aim of this work was to find the technique of enzyme immunoassay (EIA) of capillary blood lyophilized on a filter paper disk. The author has selected a buffer on the basis of egg yolk aqueous extract, that permits blocking of nonspecific binding in EIA but not preventing the specific antigen-antibody interactions. The results of analyses of capillary blood lyophilized drops and of blood serum samples, carried out in 7 tuberculous patients and 6 donors, coincided. The results were tested in 57 blood samples.