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Updated: Jul 18, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Processing of soybean products by semipurified plant and microbial alpha-galactosidases
Daniel L Falkoski1, Valéria M Guimarães, Carina M Callegari
1BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG 36.570-000, Brazil.
Abstract:
Galactooligosaccharides (GO) are responsible for intestinal disturbances following ingestion of legume-derived products. Enzymatic reduction of GO level in these products is highly desirable to improve their acceptance. For this purpose, plant and microbial semipurified alpha-galactosidases were used for GO hydrolysis in soybean flour and soy molasses. alpha-Galactosidases from soybean germinating seeds, Aspergillus terreus, and Penicillium griseoroseum presented maximal activities at pH 4.0-5.0 and 45-65 degrees C. The KM,app values determined for raffinose by the soybean, A. terreus, and P. griseoroseum alpha-galactosidases were 3.44, 19.39, and 20.67 mM, respectively. The enzymes were completely inhibited by Ag+ and Hg2+, whereas only soybean enzyme was inhibited by galactose. A. terreus alpha-galactosidase was more thermostable than the enzymes from the other two sources. This enzyme maintained about 100% of its original activity after 3 h at 60 C. The microbial alpha-galactosidases were more efficient for reducing GO in soybean flour and soy molasses than soybean enzyme.
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