Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synaptosomal protein synthesis is selectively modulated by learning.

Maria Eyman1, Carolina Cefaliello, Eugenia Ferrara

  • 1Department of Biological Sciences, University of Naples Federico II, Italy.

Brain Research
|December 21, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Fat Diet Anticipates Age-Related Sarcopenia Through Increased Oxidative Stress and Inflammation.

British journal of biomedical science·2026
Same author

Involvement of serotonin receptor 7 in synaptic dysfunctions in a mouse model of autism spectrum disorder.

European journal of pharmacology·2026
Same author

The serotonin receptor 7 as an emerging target to restore altered neuroplasticity in Angelman syndrome.

Experimental neurology·2026
Same author

Formyl peptide receptor 2 activation by MR-39 inhibits glioblastoma cell proliferation and invasiveness through suppression of multiple oncogenic pathways.

Journal of translational medicine·2026
Same author

Autism spectrum disorders and nutritional interventions: dimethylglycine and B-vitamins effects on behaviour, inflammation, microbiota and mitochondria in liver and brain synapses.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Cortico-striatal circuit mechanisms drive the effects of D1 dopamine agonists on memory capacity in mice through cAMP/PKA signalling.

Nature communications·2025

Synaptic protein synthesis in rat brains was studied in trained and control rats. Specific protein synthesis increased in trained rats, suggesting a role in brain plasticity and learning.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Behavioral Neuroscience

Background:

  • Synaptosomes are crucial for studying synaptic protein synthesis.
  • Understanding protein synthesis in brain plasticity is essential for neuroscience research.

Purpose of the Study:

  • To investigate the role of synaptic protein synthesis in brain plastic events.
  • To examine if training for an active avoidance task alters synaptic protein synthesis in specific brain regions.

Main Methods:

  • Used Ficoll-purified synaptosomes from rat neocortex, hippocampus, and cerebellum.
  • Analyzed protein synthesis capacity and specific activity in trained versus control rats.
  • Utilized SDS-PAGE and quantitative densitometry to identify and quantify specific proteins.

Related Experiment Videos

Main Results:

  • No significant difference in overall synaptosomal protein synthesis capacity between trained and control rats.
  • Selective enhancement in the synthesis of two specific proteins (66.5 kDa and 87.6 kDa) in trained rats.
  • The 66.5 kDa protein's synthesis correlated with avoidance/escape behaviors and inversely with freezing, particularly in the neocortex and cerebellum.

Conclusions:

  • Synaptic protein synthesis is involved in the plastic events of behaving rats.
  • The synthesis of a specific 66.5 kDa synaptic protein is modulated by learned avoidance responses.
  • This modulation is region-specific and linked to the reprogramming of neural circuits involved in escape and freezing behaviors.