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Related Experiment Video

Updated: Jul 7, 2026

Generation of Human CD40-activated B cells

Generation of Human CD40-activated B cells

Published on: October 16, 2009

Growing human B lymphocytes in the CD40 system.

J Banchereau¹, F Rousset

¹Schering-Plough Corporation, Laboratory for Immunological Research, Dardilly, France.

|October 17, 1991

Summary

This summary is machine-generated.

Developing effective culture techniques is crucial for generating human monoclonal antibodies. These methods enable the long-term growth of B lymphocytes, a key step facilitated by the CD40 system.

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Last Updated: Jul 7, 2026

Generation of Human CD40-activated B cells

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Published on: October 16, 2009

Murine Model of CD40-activation of B cells

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Published on: March 5, 2010

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

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Published on: August 9, 2019

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Human monoclonal antibodies are vital therapeutic agents.
Generating these antibodies often requires specific B lymphocyte culture conditions.
The CD40 system plays a role in B cell survival and proliferation.

Purpose of the Study:

To explore culture techniques for long-term B lymphocyte growth.
To investigate the role of the CD40 system in supporting B cell cultures.

Main Methods:

Development of specialized cell culture media and conditions.
Utilizing CD40 ligand or agonistic antibodies to stimulate B cells.
Monitoring B lymphocyte viability and proliferation over extended periods.

Main Results:

Successful long-term culture of a majority of B lymphocytes was achieved.
The CD40 system was identified as a critical factor for sustained B cell growth.
Culture techniques were optimized for antibody production.

Conclusions:

Long-term B lymphocyte culture is feasible using CD40-dependent methods.
This approach supports the generation of human monoclonal antibodies.
Optimized culture systems advance antibody-based therapeutics.