Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intestinal cell apoptosis and Bcl-2 expression.

Y Shaposhnikov1, Y Maheshwari, D E Sykes

  • 1Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, State University of New York, Buffalo.

Cell Death and Differentiation
|January 1, 1996
PubMed
Summary

Programmed cell death (apoptosis) is a normal process in intestinal cells. Increased Bcl-2 expression may protect proliferative enterocytes from stress-induced apoptosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Galectin-1 suppresses methamphetamine induced neuroinflammation in human brain microvascular endothelial cells: Neuroprotective role in maintaining blood brain barrier integrity.

Brain research·2015
Same author

Nanotherapeutic approach for opiate addiction using DARPP-32 gene silencing in an animal model of opiate addiction.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology·2015
Same author

Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes.

Brain research·2008
Same author

Acute esophageal necrosis: report of two cases and a review of the literature.

Gastrointestinal endoscopy·1999
Same author

Heat shock protein 70 expression in native and heterotopically transplanted rat hearts.

The Journal of surgical research·1997
Same author

Rat intestinal crypt-cell replication factor with homology to early S-phase proteins required for cell division.

Gene·1995

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Gastroenterology

Background:

  • Programmed cell death, or apoptosis, is a fundamental biological process.
  • The Bcl-2 proto-oncogene plays a key role in regulating apoptosis.
  • Enterocytes and proliferative crypt cells undergo apoptosis under normal and stress conditions.

Purpose of the Study:

  • To investigate the relationship between enterocyte apoptosis and Bcl-2 expression in the rat intestine.
  • To evaluate Bcl-2 transcript levels and DNA fragmentation in different intestinal cell fractions and cell cultures.

Main Methods:

  • Analysis of DNA fragmentation in isolated rat intestinal cell nuclei.
  • Quantification of Bcl-2 mRNA levels in rat enterocyte fractions and IEC tissue culture cells.

Related Experiment Videos

  • Induction of apoptosis in IEC cells via long-term confluent culture and serum deprivation.

    • Main Results:

    • Low levels of DNA fragmentation were observed in all enterocyte fractions, including crypt cells.
    • Detectable DNA fragmentation in IEC cells occurred under long-term culture conditions.
    • Bcl-2 mRNA was absent in isolated rat intestinal cells but present in IEC cells, increasing with serum deprivation and culture duration.

    Conclusions:

    • Increased Bcl-2 expression may be crucial for the survival of proliferative enterocytes facing stress.
    • Bcl-2 regulation might be a protective mechanism against apoptosis in intestinal epithelial cells.
    • Further research into Bcl-2's role in intestinal cell homeostasis is warranted.