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Using blood hemoglobin for blood analysis.

Vanesa Sanz1, Susana de Marcos, Javier Galbán

  • 1Analytical Biosensors Group, Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza and Institute of Nanotechnology, Zaragoza, 50009, Spain.

The Analyst
|December 21, 2006
PubMed
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This study shows that blood hemoglobin

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Spectrophotometry

Background:

  • Blood hemoglobin (Hb) spectrophotometry offers potential for direct biochemical analysis.
  • Existing methods for biochemical compound determination in blood can be complex.

Purpose of the Study:

  • To develop a novel spectrophotometric method for direct biochemical compound determination in blood using Hb.
  • To establish a theoretical model for predicting the method's applicability to various compounds.
  • To validate the method for glucose determination in blood samples.

Main Methods:

  • Developed a model correlating Hb absorbance variation with glucose concentration.
  • Prepared blood samples by dilution and azide addition to prevent interference.
  • Utilized spectrophotometry for direct analyte measurement.

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Main Results:

  • Established a linear response range for glucose determination from 2 to 540 mg/dL.
  • Achieved good correlation with an automatic analyzer for direct glucose determination.
  • Demonstrated method applicability to other compounds requiring H(2)O(2) production.

Conclusions:

  • Spectrophotometric properties of Hb enable direct determination of biochemical compounds in blood.
  • The developed method is accurate and reliable for glucose measurement, with potential for broader applications.
  • Simple sample preparation and blocking of lateral reactions are key to method success.