Related Experiment Video
Updated: Jul 18, 2026

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
Published on: September 12, 2013
Respiratory burst as a biomarker for stress responses
K Huber1, M Krötz-Fahning, B Hock
1Chair of Cell Biology, Technische Universität München, Munich, Germany. huberk@wzw.tum.de
Abstract:
A module for the detection of immunotoxic events within the test system Triple-Lux to be used during spaceflights was developed. It is based on the production of reactive oxygen species within the respiratory burst during phagocytosis or after stimulation of the phagocytes with phorbol 12-myristate 13-acetate (PMA). For this purpose, luminol-dependent chemiluminescence was measured. The assays were carried out with polymorphonuclear leukocytes purified from sheep peripheral blood. The influence of hydrocortisone and Cd2+ on the respiratory burst in polymorphonuclear leukocytes was assayed. Hydrocortisone in concentrations between 10(-4) and 10(-9) mol/liter showed an immunostimulating effect after PMA treatment. An immunosuppressive effect was observed for Cd2+ in concentrations between 10(-4) and 10(-7) mol/liter. Cryoconservation, which has often been critical for primary cells, can be accomplished without any subsequent loss of function by freezing the cells in dimethyl sulfoxide-containing medium.
Related Concept Videos
Acute Respiratory Failure-IV
Alterations in Respiration II
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes include...
Assessment of Respiration
Subjective Assessment: Nurses interview the patient to gather information directly during the subjective assessment. It includes questions about the individual's medical history, medications, and symptoms, focusing on past respiratory conditions like asthma or COPD,...
Respiratory Volumes and Capacities I
Physical Assessment of the Respiratory Tract II: Inspection
Chest Configuration
The chest configuration can...
Physiological Foundation of Stress
Role of the Sympathetic Nervous System
Adrenaline triggers the...
