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Updated: Jul 18, 2026

Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta
Published on: June 10, 2015
Effect of nitroglycerine on content of second messengers in myocytes of rat thoracic aorta
A S Dukhanin1, N L Shimanovskii
1Russian State Medical University, Moscow.
Abstract:
We evaluated the concentration dependence and time dependence of the effect of nitroglycerine on intracellular content of cAMP, cGMP, and free Ca2+. It was shown that after norepinephrine stimulation, nitroglycerine exhibited calcium-blocking activity in lower concentrations (starting from 10(-7) M). Under conditions of experimental nitrate tolerance the dose-dependent effect of nitroglycerine on intracellular cGMP and Ca2+ was less pronounced. Calcium-blocking activity of nitroglycerine decreased most significantly upon stimulation of myocytes with norepinephrine.
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