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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 18, 2026

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
12:56

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

[Scanning aneugen and clastogen by micronuclei analysis using flow cytometry].

Ming-Jie Yang1, Jian-Chang Zhou, Zhi Li

  • 1Guangdong Centre for Disease Control and Prerention, Guangzhou 510300, China.

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases
|December 22, 2006
PubMed
Summary

Flow cytometry effectively distinguishes micronuclei (MN) caused by aneugens from those caused by clastogens. This method differentiates larger MN from aneugen exposure from smaller MN from clastogen exposure in mouse models.

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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

Related Experiment Videos

Last Updated: Jul 18, 2026

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
12:56

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
09:11

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

Area of Science:

  • Genotoxicology
  • Flow Cytometry
  • Cellular Biology

Context:

  • Micronuclei (MN) are biomarkers of genotoxicity.
  • Distinguishing aneugen-induced (larger) from clastogen-induced (smaller) MN is crucial for understanding DNA damage mechanisms.
  • Current methods for MN analysis can be labor-intensive and lack specificity.

Purpose:

  • To develop and validate a flow cytometry (FCM)-based assay for discriminating aneugen- and clastogen-induced micronuclei.
  • To utilize fluorescently labeled antibodies and DNA stains for quantitative MN analysis.
  • To assess the utility of FCM in differentiating MN size based on the causative agent.

Summary:

  • A novel flow cytometry method was established using anti-CD71-FITC and propidium iodide (PI) staining.
  • Peripheral blood micronucleated reticulocytes (MN-RET) in NIH mice treated with colchicine (aneugen) or cyclophosphamide (clastogen) were analyzed.
  • The ratio of MN-RET fluorescent signal intensity to nucleated cell intensity was significantly higher for colchicine-treated mice (aneugen) and lower for cyclophosphamide-treated mice (clastogen).

Impact:

  • This FCM assay provides a rapid and quantitative method for distinguishing aneugen- from clastogen-induced micronuclei.
  • The findings support the use of FCM for mechanistic genotoxicity testing.
  • Enables better discrimination of DNA damage types in toxicological studies.