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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Vibrio cholerae strain typing and phylogeny study based on simple sequence repeats
Yael Danin-Poleg1, Lyora A Cohen, Hanan Gancz
1Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Journal of Clinical Microbiology
|December 22, 2006
Summary
A new method using simple sequence repeats (SSR) and mononucleotide repeats (MNR) accurately types Vibrio cholerae strains. This approach offers rapid discrimination between clinical and environmental isolates, improving cholera surveillance.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- Vibrio cholerae causes cholera, with its reservoir in aquatic environments.
- Current V. cholerae typing relies on serology, requiring extensive antisera.
- Simple sequence repeats (SSR) and mononucleotide repeats (MNR) offer genomic polymorphism for bacterial typing.
Purpose of the Study:
- To develop and evaluate an accurate and rapid V. cholerae typing method using SSR and MNR loci.
- To assess the discriminatory power of SSR and MNR variations for distinguishing clinical and environmental strains.
Main Methods:
- In silico identification of SSRs in the V. cholerae genome.
- Analysis of polymorphism in long SSR (L-SSR) loci using capillary fragment-size analysis.
- Assessment of MNR polymorphism and single nucleotide polymorphisms (SNPs) in flanking sequences via sequencing (MNR-MLST).
Main Results:
- Thousands of perfect SSR tracts were identified in the V. cholerae genome.
- All nine tested L-SSR loci were polymorphic, with 2-13 alleles each.
- Combined L-SSR and MNR-MLST typing achieved discrimination among 27 of 32 V. cholerae strains, clearly separating O1/O139 clinical strains from environmental isolates.
Conclusions:
- SSR-based typing, combining L-SSR and MNR-MLST, is efficient for V. cholerae typing.
- This method provides accurate and rapid discrimination of V. cholerae strains.
- The approach enhances capabilities for V. cholerae surveillance and epidemiological studies.
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