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Updated: Jul 18, 2026
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
Published on: April 14, 2015
Fluorescent multiplex amplification of three X-STR loci
Qiu-Ling Liu1, De-Jian Lü, Jia-Zhen Zhu
1Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China.
This study evaluated three X-STR loci (DXS6803, DXS981, DXS6809) for forensic use. The multiplex system showed high polymorphism, proving valuable for identification and paternity testing.
Area of Science:
- Forensic Genetics
- Molecular Biology
Background:
- X-STR loci are crucial for forensic DNA analysis.
- Evaluating novel X-STR loci enhances identification capabilities.
Purpose of the Study:
- To assess the forensic value of three X-STR loci (DXS6803, DXS981, DXS6809).
- To investigate the polymorphism of these X-STR loci in the Han population of China.
Main Methods:
- Developed a fluorescent multiplex PCR assay for simultaneous amplification of three X-STR loci.
- Analyzed PCR products using capillary electrophoresis on an ABI PRISM 3100 Genetic Analyzer.
Main Results:
- Observed 13, 12, and 11 alleles for DXS6803, DXS981, and DXS6809, respectively.
- Detected 183 unique haplotypes in males with a haplotype diversity of 0.9926.
- Demonstrated significant polymorphic information content for forensic applications.
Conclusions:
- The evaluated X-STR multiplex system offers high discriminatory power for forensic identification.
- This system is particularly useful for complex paternity testing scenarios.
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