Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human plasma ATP concentration.

Mark W Gorman1, Eric O Feigl, Charles W Buffington

  • 1Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195-7290, USA. mgorman@u.washington.edu

Clinical Chemistry
|December 23, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of myocardial oxygen demand on the coronary vascular response to arterial blood gas changes in humans.

American journal of physiology. Heart and circulatory physiology·2018
Same author

"Loose Lips Are Actually Good".

Anesthesiology·2017
Same author

Adverse Drug Events Link to Severity of the Event Data Needed.

Anesthesiology·2016
Same author

Open-loop (feed-forward) and feedback control of coronary blood flow during exercise, cardiac pacing, and pressure changes.

American journal of physiology. Heart and circulatory physiology·2016
Same author

A Tool for Assuring Continuity of Care During a Series of ECT Treatments.

The journal of ECT·2015
Same author

CrossTalk opposing view: Guyton's venous return curves should not be taught.

The Journal of physiology·2013
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
Same journal

Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

Clinical chemistry·2026
Same journal

In Reply to Reflexing NT-proBNP for sFlt-1/PlGF Ratios That Fall into the Measurement Uncertainty for Preeclampsia Risk Classification.

Clinical chemistry·2026
See all related articles

Human plasma adenosine triphosphate (ATP) levels are significantly lower than previously reported when blood sample processing inhibits ATP release from blood cells. Aspirin pretreatment did not affect ATP concentrations.

Area of Science:

  • Biochemistry
  • Physiology

Background:

  • Human plasma adenosine triphosphate (ATP) concentration is widely reported around 1000 nmol/L.
  • This study investigated whether inhibiting ATP release during blood sample processing affects measured plasma ATP levels.

Purpose of the Study:

  • To test if inhibiting ATP release from blood elements during sample processing lowers measured plasma ATP concentration.
  • To determine if aspirin pretreatment reduces plasma ATP concentration by inhibiting platelet activity.

Main Methods:

  • Blood samples were collected from 20 healthy individuals pretreated with aspirin or placebo.
  • Samples were processed using either standard EDTA/saline or a novel stabilizing solution to inhibit ATP catabolism and release.
  • Plasma ATP was quantified using the luciferin-luciferase assay and corrected for hemolysis.

Related Experiment Videos

Main Results:

  • Aspirin pretreatment showed no significant impact on plasma ATP concentrations.
  • The stabilizing solution yielded mean ATP concentrations 8-fold lower (28 nmol/L) compared to EDTA alone (236 nmol/L).

Conclusions:

  • Human venous plasma ATP concentration is substantially lower than previously estimated when ATP release during sample preparation is inhibited.
  • The findings highlight the importance of sample handling techniques in accurately determining plasma ATP levels.