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Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Transposable element Ds2 of Zea mays influences polyadenylation and splice site selection
1Institut für Genetik, Universität zu Köln, D-5000 Köln 41, Federal Republic of Germany.
Abstract:
In the allele adh1-2F11 of Zea mays the 1.3 kb transposable element Ds2 is inserted in the fourth exon of Adh1. Two major RNAs of 3.0 and 1.6 kb are transcribed from this allele. While the 3.0 kb transcript also contains the Ds2 sequences, the 1.6 kb transcript has lost the Ds2 sequences by an alternative splicing process. In this process, a normal 5' splice site is joined to a cryptic 3' splice site located within exon 4. This cryptic 3' splice site is not used in a wild-type Adh1F allele. Other minor transcripts of adh1-2F11 are prematurely terminated and polyadenylated.
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