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Detection and Isolation of Campylobacter spp. from Raw Meat
Published on: February 23, 2024
Distribution of Campylobacter genotypes on broilers during slaughter.
H Lindmark1, I C Diedrich, L Andersson
1National Food Administration, P.O. Box 622, SE-751 26, Uppsala, Sweden. hlin@slv.se
Journal of Food Protection
|December 26, 2006
Summary
Genotyping methods reveal Campylobacter contamination on chicken carcasses. Most genotypes found on carcasses originate from the same slaughter group or earlier, indicating survival through processing.
Area of Science:
- Food safety
- Microbiology
- Veterinary science
Background:
- Campylobacter is a leading cause of bacterial gastroenteritis globally.
- Effective genotyping is crucial for understanding Campylobacter transmission dynamics in poultry.
- Poultry carcasses are a significant reservoir for Campylobacter transmission to humans.
Purpose of the Study:
- To investigate the frequency and diversity of Campylobacter genotypes on chicken carcasses post-slaughter.
- To determine the origin and transmission routes of Campylobacter genotypes in a poultry processing environment.
- To assess the survival of Campylobacter genotypes during poultry processing.
Main Methods:
- Macrorestriction profiling was used to subtype Campylobacter isolates.
- Isolates were recovered from chicken carcasses (post-chilling), neck skin, and cloacal swabs.
- Analysis included isolates recovered with and without enrichment from carcasses.
Main Results:
- An average of 1.5 Campylobacter genotypes were found per carcass.
- Carcass genotypes largely matched cloacal samples from the same or preceding slaughter groups.
- Most cloacal genotypes were detected on corresponding carcasses, indicating survival through processing.
Conclusions:
- Chicken carcasses are typically contaminated with one or two distinct Campylobacter genotypes.
- Campylobacter transmission can occur between slaughter groups, highlighting the need for stringent hygiene protocols.
- Genotyping provides valuable insights into the sources and spread of Campylobacter in poultry production.
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