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Mapping the 5' ends of banana bunchy top virus gene transcripts
V A Herrera-Valencia1, B Dugdale, R M Harding
1Centre for Tropical Crops and Biocommodities, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
Abstract:
Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3' rapid amplification of cDNA ends (3' RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5' cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.
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