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Updated: Jul 18, 2026

Induction and Validation of Cellular Senescence in Primary Human Cells
Published on: June 20, 2018
P2X7-induced apoptosis decreases by aging in mice myeloblasts
Edgar Julian Paredes-Gamero1, Juliana Luporini Dreyfuss, Helena B Nader
1Department of Biophysics, Federal University of São Paulo, R. Botucatu 862, Brazil. jean@biofis.epm.br <jean@biofis.epm.br>
Abstract:
In the current study, the ability of ATP to promote apoptosis in myeloblasts at different ages was investigated. We have observed that high concentration of extracellular ATP (>1mM), which activates P2X(7) receptor, produced cell shrinkage an increase in the number of events in the sub-G(0)/G(1) region of the cellular cycle and annexin-V/propidium iodide label, which characterizes the apoptotic cell death. In addition, BzATP produced apoptosis, but not ADP and UTP. Gr-1(+) cells express the P2X(7) receptor and oxidized ATP, a specific P2X(7) inhibitor, blocked the ATP-dependent apoptosis. ATP-dependent apoptosis is decreased by aging in myeloblasts of 12 and 22-month-old mice. Furthermore, P2X(7) expression decrease was observed in older mice, explaining apoptosis decrease. This decrease in apoptosis by aging may be related to some diseases in the myelocyte lineage.
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