Related Experiment Video
Updated: Jul 18, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand
Michiei Oto1, Wataru Suda, Hirofumi Shinoyama
1Department of Biotechnology, Tokyo Technical College, Higashi, Kunitachi-shi, Tokyo 186-0002, Japan. ooto@tera-house.ac.jp
Abstract:
We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.
Related Concept Videos
Modern Molecular Taxonomy
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

