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Related Experiment Videos

Rugosity in Grimontia hollisae.

S K Curtis1, M H Kothary, R J Blodgett

  • 1U.S. Food and Drug Administration, College Park, MD 20740, USA.

Applied and Environmental Microbiology
|December 26, 2006
PubMed
Summary
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Grimontia hollisae exhibits rugose and smooth colony variants. Rugose colonies, producing more exopolysaccharide and biofilm, show increased stress resistance and temperature-dependent morphology changes.

Area of Science:

  • Microbiology
  • Bacteriology
  • Cell Biology

Background:

  • Grimontia hollisae (formerly Vibrio hollisae) presents distinct colonial phenotypes: smooth and rugose.
  • The rugose phenotype is associated with wrinkled colonies and abundant exopolysaccharide production.

Purpose of the Study:

  • To characterize the rugose colonial phenotype of Grimontia hollisae.
  • To investigate the influence of growth temperature on colony morphology and exopolysaccharide expression.
  • To assess the stress response and biofilm formation capabilities of rugose versus smooth variants.

Main Methods:

  • Colony morphology observation and characterization.
  • Flow cytometry for cell clumping and lectin binding analysis (wheat germ agglutinin, Galanthus nivalis lectin).

Related Experiment Videos

  • Biofilm assays and ultrastructural analysis using electron microscopy.
  • Stress survival assays (bleach exposure) and suckling mouse infection models.

    • Main Results:

    • Rugose colonies exhibit temperature-dependent morphology changes, forming rugose colonies at 30°C and smooth colonies at 37°C.
    • Rugose cells showed increased resistance to bleach stress, greater clumping, and higher binding of specific lectins (WGA, GNL), indicating N-acetylglucosamine and mannose in exopolysaccharide.
    • Rugose colonies produced significantly more biofilm material than smooth colonies, with higher production at 30°C.
    • Ultrastructural analysis revealed distinct cellular differentiation and matrix production in rugose colonies, absent in smooth colonies.

    Conclusions:

    • This study provides the first detailed characterization of rugosity in G. hollisae.
    • Exopolysaccharide production and rugose phenotype expression are influenced by growth temperature.
    • The rugose phenotype confers advantages in stress resistance and biofilm formation.