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Updated: Jul 18, 2026

Studying Muscle Transcriptional Dynamics at Single-molecule Scales in Drosophila
Published on: September 8, 2023
Musclin gene expression is strongly related to fast-glycolytic phenotype
S Banzet1, N Koulmann, H Sanchez
1Centre de Recherches du Service de Santé des Armées, Department of Human Factors, 24 avenue des maquis du Grésivaudan, BP 87, 38702 La Tronche Cedex, France. sbanzet@crssa.net
Abstract:
Musclin has been described as a muscle-derived secretory peptide, responsive to insulin in vivo, and inducing insulin resistance in vitro. Because muscle fibers display very different metabolic properties and insulin sensitivity, we tested the hypothesis that musclin expression could depend on myofiber type. Musclin mRNA was detected at high level in fast gastrocnemius and plantaris muscles, but only as traces in soleus, a slow-twitch muscle. A single fiber analysis showed that musclin was produced by muscle fibers themselves, almost exclusively type IIb fibers. Slow to fast transition of soleus phenotype after hindlimb suspension increased musclin mRNA levels, whereas fast to slow transition of plantaris phenotype after functional overload decreased musclin mRNA levels. This clearly suggests that musclin transcription is strongly related to fast-glycolytic phenotype. We conclude that musclin is produced by myocytes in a highly fiber-type specific manner and that physiological changes in type IIb MHC lead to coordinated musclin expression.
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