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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
Published on: May 30, 2014
Fluorescent measurement of microalgal neutral lipids
Danielle Elsey1, David Jameson, Barry Raleigh
1Hawaii Natural Energy Institute, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1680 East-West Road, POST 109, Honolulu, Hawaii 96822, United States.
Journal of Microbiological Methods
|December 27, 2006
Abstract:
Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of the surrounding medium, has been proposed to determine the neutral lipid content of microalgal cells. Herein we communicate modifications to this technique that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility.
