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Updated: Jul 18, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
Published on: February 7, 2021
Hydrolytic cleavage of DNA by quercetin zinc(II) complex
Tan Jun1, Wang Bochu, Zhu Liancai
1Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, PR China. tanjunmail@126.com
Abstract:
Quercetin zinc(II) complex was investigated focusing on its hydrolytic activity toward DNA. The complex successfully promotes the cleavage of plasmid DNA, producing single and double DNA strand breaks. The amount of conversion of supercoiled form (SC) of plasmid to the nicked circular form (NC) depends on the concentration of the complex as well as the duration of incubation of the complex with DNA. The rate of conversion of SC to NC is 1.68x10(-4) s(-1) at pH 7.2 in the presence of 100 microM of the complex. The hydrolytic cleavage of DNA by the complex is supported by the evidence from free radical quenching, thiobarbituric acid-reactive substances (TBARS) assay, and T4 ligase ligation.
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