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Updated: Jul 18, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
[Analysis of 37 drugs in whole blood by HPLC after solid phase extraction]
Chen Liang1, Yu-Rong Zhang, Qi-Yun Jin
1Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
Objective:
To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.
Methods:
With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.
Results:
The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.
Conclusion:
The method is effective, simple, reliable and has been used in real cases.
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