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[Study of Papaver somniferum cultivars identification by TD-RAPD technique]
1Yunnan Criminal Science and Technology Institute, Kunming 650021, China.
Objective:
The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.
Method:
Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.
Result:
We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.
Conclusion:
TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
