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Updated: Jul 18, 2026
Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
Published on: June 4, 2014
Full developmental potential of mammalian preimplantation embryos is maintained after imaging using a spinning-disk
Pablo Juan Ross1, Gloria Ines Perez, Tak Ko
1The Cellular Reprogramming Laboratory, Michigan State University, East Lansing, MI, USA.
Abstract:
Fluorescent live imaging of cells and embryos at subcellular resolution poses significant challenges for biologists due to morbidity and mortality ensuing from phototoxicity. Here we report the use of a spinning-disk confocal microscope to image mouse and bovine preimplantation embryos without impairing their developmental potential. We also present data indicating that this imaging technique does not affect the functionality of subcellular components as assessed by reactive oxygen species (ROS) production, caspase activity, and DNA integrity. Spinning-disk confocal microscopy was also useful in determining cell number and allocation in transgenic bovine blastocysts. We conclude that this imaging method is suitable for monitoring preimplantation embryos.
Insights
Spinning-disk confocal microscopy enables live imaging of preimplantation embryos without harming their development. This advanced technique preserves subcellular functions and is ideal for monitoring embryo development.
Area of Science:
- Developmental biology
- Cell biology
- Microscopy
Background:
- Phototoxicity from fluorescent live imaging can harm cells and embryos.
- Subcellular resolution imaging is crucial but challenging due to potential damage.
Purpose of the Study:
- To evaluate spinning-disk confocal microscopy for imaging preimplantation embryos.
- To assess the impact of this technique on embryo development and subcellular function.
Main Methods:
- Utilized spinning-disk confocal microscopy to image mouse and bovine preimplantation embryos.
- Assessed embryo developmental potential post-imaging.
- Measured reactive oxygen species (ROS) production, caspase activity, and DNA integrity.
- Determined cell number and allocation in transgenic bovine blastocysts.
Main Results:
- Spinning-disk confocal microscopy did not impair the developmental potential of preimplantation embryos.
- Imaging did not negatively affect subcellular components, including ROS production, caspase activity, and DNA integrity.
- The technique successfully allowed for cell counting and allocation analysis in blastocysts.
Conclusions:
- Spinning-disk confocal microscopy is a suitable method for monitoring preimplantation embryo development.
- This technique minimizes phototoxicity, preserving embryo viability and function.
- It offers a valuable tool for live imaging in developmental and cell biology research.
