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Updated: Jul 18, 2026

A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
Published on: August 11, 2014
Reduced apoptosis in human intestinal cells cured of persistent poliovirus infection
Karine Labadie1, Aure Saulnier, Sandra Martin-Latil
1Virus Entérotropes et Stratégies Antivirales, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.
Abstract:
Cells cured of persistent virus infection can be used to investigate cellular pathways of resistance to viral cytopathic effects. Persistent poliovirus (PV) infections were established in human intestinal Caco-2 cells, and spontaneously cured cell cultures were obtained. Two cell clones, cl6 and b13, cured of type 3 PV mutant infection and their parental Caco-2 cells were compared for susceptibility to PV infection, PV receptor CD155 expression, capacity to differentiate into polarized enterocytes, and PV-, staurosporine-, and actinomycin D-induced apoptosis. Our results strongly suggest that cells that are partially resistant to apoptosis can be selected during persistent virus infection.
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