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Related Concept Videos

Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
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Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
Microtubules in Cell Motility01:24

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Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Related Experiment Video

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Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
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Lack of adenomatous polyposis coli protein correlates with a decrease in cell migration and overall changes in

Karin Kroboth1, Ian P Newton, Katsuhiro Kita

  • 1Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.

Molecular Biology of the Cell
|December 29, 2006
PubMed
Summary

Loss of the adenomatous polyposis coli (APC) gene impairs cell migration and microtubule stability, crucial functions in gut tissue. This discovery highlights a vulnerability in APC-deficient cells for potential cancer therapies.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Most sporadic colorectal tumors have APC gene mutations.
  • The APC protein regulates beta-catenin and microtubules, impacting gut tissue.
  • Truncation mutations in APC disrupt microtubule binding, potentially causing cellular defects.

Purpose of the Study:

  • To investigate the role of APC in cell migration and microtubule stability.
  • To determine the consequences of APC loss on cellular protrusions and cell movement.
  • To explore the therapeutic potential of targeting APC-deficient cells.

Main Methods:

  • Studied the effects of APC loss on cellular protrusions and migration.
  • Assessed overall microtubule stability and peripheral microtubule modifications.
  • Examined the impact of APC overexpression on cell shape and protrusions.

Main Results:

  • Loss of APC leads to the disappearance of cellular protrusions and reduced cell migration.
  • Microtubule stability decreases, with fewer posttranslationally modified microtubules at the cell periphery.
  • Overexpression of APC can induce cellular protrusions, affecting cell shape.

Conclusions:

  • Cell migration and microtubule stability are directly linked to APC status.
  • APC deficiency causes specific cellular defects, including impaired migration and altered microtubule dynamics.
  • Targeting APC-deficient cells represents a potential therapeutic strategy for colorectal cancer.