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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

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Tone reversal of an AFM lateral force image due to hybridization of oligonucleotides immobilized on polymers

Dan V Nicolau1, Duy K Pham, Elena P Ivanova

  • 1Faculty of Engineering & Industrial Sciences, Swinburne University of Technology, Hawthorn, Victoria, 3122, Australia. dnicolau@swin.edu.au

Small (Weinheim an Der Bergstrasse, Germany)
|December 29, 2006
PubMed
Abstract

No abstract available in PubMed .

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Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...