Related Experiment Video
Updated: Aug 14, 2026

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
Published on: October 29, 2012
Galanin stimulates acetylcholine release in the rat striatum
1Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Abstract:
The effect of the neuropeptide galanin (GAL) on the basal and the evoked release of acetylcholine (ACh) was investigated in the rat striatum using microdialysis and HPLC techniques. GAL (0.3, 1 and 3 nmol), applied in the lateral ventricle (10 microliters), was found to cause a dose-dependent stimulation of the basal ACh release. The stimulating effect of GAL on ACh release was longlasting (greater than 90 min) and reached its peak 30 min after i.c.v. administration. GAL failed to affect the scopolamine (0.25 and 0.5 mg/kg, i.p.) stimulated release of ACh. Possible mechanisms behind the GAL-stimulated ACh release in the rat striatum are discussed. It may involve effects on GAL receptors in the striatum or indirect effects via stimulation of GAL receptors in the substantia nigra resulting in inhibition of striatal dopamine (DA) transmission.
More Related Videos
09:36Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
Published on: August 13, 2017
10:46Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats
Published on: June 22, 2017
Related Concept Videos
Cholinergic Neurons: Neurotransmission
Cholinergic Receptors: Muscarinic
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...
Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
The direct-acting...
Direct-Acting Cholinergic Agonists: Pharmacokinetics
Indirect-Acting Cholinergic Agonists: Pharmacological Actions
At the neuromuscular junction, these agents work by inhibiting the breakdown of acetylcholine, allowing it to remain bound to the receptor and bind to nearby receptors. This process leads to repetitive firing of the endplate, causing muscle...
Drugs Acting on Autonomic Ganglia: Stimulants
Ganglionic stimulants activate NM nicotinic receptors in autonomic ganglia, falling into two categories: nicotine mimetics [e.g., lobeline, dimethylpiperazine, tetramethylammonium] and muscarinic receptor agonists [e.g., muscarine, methacholine]. The first category's action is rapid and blocked by nicotinic receptor antagonists, while the second category's action is delayed and blocked by atropine-like agents. Nicotine, an alkaloid, affects the heart rate by stimulating sympathetic or...