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Human papillomavirus detection in urine samples: an alternative screening method
Francesc Alameda1, Beatriz Bellosillo, Pere Fusté
1Department of Pathology, University Hospital del Mar, Autonomous University of Barcelona, Barcelona, Catalonia, Spain. 86780@imas.imim.es
Journal of Lower Genital Tract Disease
|December 30, 2006
Summary
Human papillomavirus (HPV) detection in urine offers a promising alternative for screening women with limited gynecologic care. Urine HPV testing demonstrates high sensitivity and negative predictive value for detecting high-grade squamous intraepithelial lesions.
Area of Science:
- Gynecology
- Virology
- Molecular Diagnostics
Background:
- Cervical cancer screening is crucial for early detection and prevention.
- Access to regular gynecologic care can be a barrier for some women.
- Human papillomavirus (HPV) is the primary cause of cervical cancer.
Purpose of the Study:
- To evaluate the efficacy of detecting HPV in urine samples.
- To assess HPV detection in urine as a potential screening tool for women with poor gynecologic attention.
Main Methods:
- Collected and analyzed 50 urine and 50 cervical samples from 50 women.
- Utilized polymerase chain reaction (PCR) with consensus primers for HPV detection.
- Employed a low-density microarray for HPV typing.
Main Results:
- Achieved 80% concordance between urine and cervical sample results.
- Urine HPV PCR demonstrated 100% sensitivity and 80% specificity for high-grade squamous intraepithelial lesions.
- Reported a positive predictive value of 91% and a negative predictive value of 100% for urine samples.
Conclusions:
- HPV detection in urine samples is a viable alternative screening method.
- Urine-based HPV testing can improve screening accessibility for underserved populations.
- This approach may enhance early detection of cervical abnormalities.
