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Liver Regeneration

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The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
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Updated: Jul 18, 2026

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

Integration of technologies for hepatic tissue engineering.

Yaakov Nahmias¹, Francois Berthiaume, Martin L Yarmush

¹Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital, Harvard Medical School, 51 Blossom St, Boston, MA 02114, USA.

Advances in Biochemical Engineering/Biotechnology

|January 2, 2007

Summary

Hepatic tissue engineering advances bioartificial liver systems for treating liver failure and studying liver diseases. Innovations in hepatocyte culture and microfluidics offer new avenues for liver regeneration and therapeutic development.

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Area of Science:

Biomedical Engineering
Hepatology
Tissue Engineering

Background:

The liver performs over 500 vital functions, and its failure causes significant mortality.
Hepatic tissue engineering aims to create bioartificial liver systems for therapeutic and research applications.

Purpose of the Study:

To overview current hepatic tissue engineering techniques.
To discuss advancements in hepatocyte culture and bioreactor design.
To explore challenges and opportunities in developing novel liver support systems.

Main Methods:

Review of isolated perfused liver systems, liver slice/explant cultures, and various hepatocyte culture methods (e.g., "sandwich" cultures, spheroids).
Integration of culture techniques with microfluidics and bioreactor design.
Discussion of critical factors like oxygen and medium composition for hepatocyte function.

Main Results:

Current state-of-the-art in bioartificial liver systems and hepatocyte culture techniques are presented.
Emerging technologies for enhancing hepatocyte proliferation and function are highlighted.
The relationship between liver biology and culture system design is explored.

Conclusions:

Integrating cell culture, bioreactor design, and microtechnology is crucial for developing advanced liver support systems.
Significant challenges remain in achieving long-term hepatocyte function and clinical translation.
Future research holds promise for novel applications in liver disease treatment and research.