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Updated: Jul 18, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots
Gilles Innocenti1, Chiara Pucciariello, Marie Le Gleuher
1Interactions Plantes-Microorganismes et Santé Végétale, UMR INRA 1064/Université de Nice-Sophia Antipolis/CNRS 6192, 400, route des Chappes, BP 167, 06903, Sophia-Antipolis, France.
Abstract:
Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (gamma-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that gamma-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.
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