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Updated: Jul 18, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Fungi recovered from Slovakian poultry feed mixtures and their toxinogenity
1Microbiology Department, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic. Roman.Labuda@uniag.sk
Abstract:
To contribute towards the knowledge of microbiology of feeds, more than 100 samples of poultry feed mixtures from Slovakia were mycologically investigated in terms of the overall fungal diversity and toxicological potential of isolated fungi. The study revealed that out of 22 genera recovered, Penicillium was the most frequent and diverse genus, followed by Aspergillus and Mucor being found in 89 % (34 spp.), 69 % (11 spp.) and 50 % (4 spp.), respectively. The most frequently encountered taxa were Fusarium proliferatum, followed by Penicillium aurantiogriseum, Mucor racemosus, Penicillium crustosum and Aspergillus flavus . In addition, the following genera were recorded (in descending order) Rhizopus (44 %, 3 spp.), Eurotium (42 %, 5 spp.), Fusarium (42 %, 3 spp.), Cladosporium (31 %, 1 sp.), Alternaria (22 %, 3 spp.), Absidia (16 %, 3 spp.), Acremonium (12 %, 2 spp.), Scopulariopsis (10 %, 2 spp.), Paecilomyces (4 %, 1 sp.), Ulocladium (3 %, 1 sp.), Trichoderma (2 %, 1 sp.), Zygorrhynchus (2 %, 1 sp.), and finally Emericella, Epicoccum, Geosmithia, Monascus, Stachybotrys, Syncephalastrum and Wardomyces , all were encountered in 1 % of the samples being represented by a single species. The mean value counts of total fungi ranged from 1 x 10 (3) to 200 x 10(5) cfu/g. Outcomes from mycotoxin screening within the appropriate potentially toxinogenic species showed a number of mycotoxin producers, namely those forming aflatoxin B (1) (n=3), citrinin (17), cyclopiazonic acid (76), fumonisin B(1) (86), griseofulvin (42), moniliformin (18), ochratoxin A (5), patulin (56), penitrem A (30) and sterigmatocystin (10).
Insights
Poultry feed in Slovakia harbors diverse fungi, with Penicillium, Aspergillus, and Mucor being most common. Many isolated fungi can produce harmful mycotoxins, highlighting feed safety concerns.
Area of Science:
- Mycology
- Food Microbiology
- Toxicology
Background:
- Microbial contamination of animal feed poses risks to animal health and food safety.
- Understanding fungal diversity and mycotoxin production in poultry feed is crucial for risk assessment.
Purpose of the Study:
- To investigate the fungal diversity in Slovakian poultry feed.
- To assess the mycotoxicological potential of isolated fungal species.
Main Methods:
- Mycological analysis of over 100 poultry feed samples.
- Identification of fungal genera and species.
- Screening for mycotoxin production in potentially toxigenic isolates.
Main Results:
- Penicillium, Aspergillus, and Mucor were the most prevalent fungal genera.
- Key species identified include Fusarium proliferatum, Penicillium aurantiogriseum, and Aspergillus flavus.
- Numerous isolates demonstrated the capacity to produce mycotoxins such as aflatoxin B1, fumonisin B1, and patulin.
Conclusions:
- Slovakian poultry feed is frequently contaminated with diverse fungal species.
- A significant proportion of these fungi are capable of producing various mycotoxins.
- Findings underscore the need for stringent monitoring and control of fungal contamination in poultry feed.
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