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Updated: Jul 18, 2026
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
Published on: September 7, 2012
Tetraphenylphosphonium-selective electrode as a tool for evaluating mitochondrial permeability transition pore
A Lábajová1, J Kofránek, P Kriváková
1Institute of Pathophysiology, 1st Faculty of Medicine, Charles University, U Nemocnice 5, 120 00 Prague 2, Czech Republic. Anna.Labajova@lf1.cuni.cz
Abstract:
The changes in mitochondrial membrane potential (Deltapsi(m)) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca(2+)-induced Deltapsi(m) dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide.
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